EPIK Stem Cell miRNA Panel (BIO-66036)

BIO-66036

BIOLINE Ltd.

Dostawca: BLIRT S.A.

Cena netto, nie zawiera podatku VAT.

Użyty przelicznik: 1 euro = 4,25 zł

4 420,00 zł

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UWAGA: Zestaw zawiera wszystkie niezbędne odczynniki (m.in. odczynniki do odwrotnej transkrypcji i reakcji Real-Time PCR)!

Description

Using advanced design concepts, a proprietary algorithm has been developed by MiRXES™ to create modified stem-loop miRNA specific reverse transcription primers and hemi-nested real-time PCR primer combinations, to maximize miRNA detection sensitivity while minimizing non-specific interactions. The assays use SYBR® Green rather than a probe-based system for detection, allowing rapid amplification when required. The resulting real-time PCR assays use SensiSMART™ enabling detection of extremely low levels of miRNA with high specificity, allowing the discrimination between closely related miRNA sequences.

Performance of these assays have been validated to detect as few as 100 copies of template per RT reaction with excellent assay efficiency and linearity, to produce complete systems for miRNA profiling of stem cells.

The EPIK Stem Cell miRNA Panel Assay is made up of the most commonly studied 176 miRNAs in miRNA stem cell research (see plates). A set of controls present on this assay along with spike RNA enables data analysis, assessment of reverse transcription performance and assessment of PCR performance. The panel is designed to help in the understanding the critical roles of miRNAs in induction, maintenance, differentiation and reprogramming of stem cells helps to harness the great potential of stem cells as regenerative medicine.

EPIK miRNA Panel Assays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.


If you need single assays, please contact Bioline Technical Support for advice.

EPIK miRNA Assays Movie / Dr. Simon Baker

https://www.youtube.com/watch?v=wFGfB-MjgwU

 

Features & Benefits

 

- increased sensitivity RT and qPCR steps optimized to drive highly efficient amplification from limiting amounts (≥10pg) of total RNA
- improved specificity pre-designed, novel, miRNA-specific primers for superior discrimination of even very closely related targets
- faster protocol RNA to Ct in less than 2 hours for earlier results and increased throughput capacity
- Reliable data 7-log linear dynamic range for accurate quantification of low- and high- expressed targets in even limited sample volumes
- convenience all necessary components included in the kit and formulated to minimize set-up time

 

Instrument compatibility

 

Lo-ROX Plate 0.2Y (BIO-66036) = ABI 96-Well Block (0.2 ml) which is compatible with ABI ViiA7; ABI 7500; QuantStudio™ 3 Real-Time PCR System; QuantStudio™ 5 Real-Time PCR System; QuantStudio™ 6 Real-Time PCR system; QuantStudio™ 7 Real-Time PCR system; QuantStudio™ 12K Flex Real-Time PCR system.

Components

 

Stem Cell Assay Plates: 2x 2 96 well plates
RT Primer Pool - lyophilized: 2 tubes (A and B)
RNA Spike - lyophilized: 1 tube
EPIK RT Buffer: 32 µl
EPIK RT Enzyme: 8 µl
2 x SensiSMART™ Master Mix: 4x 1 ml
DEPC Water: 3x 1.8 ml

List of Stem Cell miRNAs on Plates

http://www.bioline.com/list-of-stemcell-panel-plates#tab1

Storage & Stability

 

All components are shipped on dry/blue ice and should be stored at -20°C upon receipt for optimum stability and the RNA Spike stored at -80°C after reconstitution. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.

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EPIK Stem Cell miRNA Panel (BIO-66036)

EPIK Stem Cell miRNA Panel (BIO-66036)

Dostawca: BLIRT S.A.

Cena netto, nie zawiera podatku VAT.

Użyty przelicznik: 1 euro = 4,25 zł

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