ACCUZYME DNA Polymerase (BIO-21051), 250 U

BIO-21051

BIOLINE Ltd.

Dostawca: BLIRT S.A.

Cena netto, nie zawiera podatku VAT.

Użyty przelicznik: 1 euro = 4,25 zł

769,25 zł

Uwaga!!! W CELU UZYSKANIA WIĄŻĄCEJ OFERTY SKONTAKTUJ SIĘ Z DOSTAWCĄ PRODUKTU- BLIRT S.A.

PROSZĘ O OFERTĘ

- High-fidelity coupled with high yield

- Amplifies fragments up to 5 Kb

- Available as a convenient pre-mixed, pre-optimized solution (ACCUZYME™ Mix)

 

Applications

- Ultra-high fidelity for subsequent cloning

- Blunt-end cloning

 

Description

ACCUZYME™ is a thermostable enzyme possessing 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities, offering high fidelity. ACCUZYME provides high fidelity with an error-rate of 3.0 x 10-6, to give blunt-ended amplicons of up to 5Kb in length.


ACCUZYME is supplied with 10x Reaction Buffer containing MgSO4, which provides optimal final reaction conditions (1.5mM Mg2+) for most experiments. In order to allow optimization of reaction conditions, additional MgCl2 is provided.


The specificity and performance of ACCUZYME can be further improved with the use of 2x PolyMate Additive, which is designed for GC or AT-rich DNA, "dirty" templates or sequences with a high level of secondary structure.

 

Unit Definition

One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.

 

Concentration

2.5u/µl

 

Storage Conditions

ACCUZYME can be stored for 12 months at -20°C.

 

Shipping Conditions

On Dry Ice or Blue Ice

 

Product Citations

1. Kitazono, A.A., Gene doi:10.1016/j.gene.2011.06.006 (2011).

2. Batchelor, D.J., et al. Am. J. Physiol. 300, R67-R75 (2011).

3. Chiang, C., et al. J. Bacteriol. 193, 52-62 (2011).

4. Lim, C.G., et al. Appl. Envir. Microbiol. 77, 3451-3460 (2011).

5. Cheng, C., et al. Mol. Cell. Biol. 31, 983-997 (2011).

6. Chakrabarti, M., et al. Virol. J. 7, 181 (2010).

7. Silvestrini, F., et al. Mol.Cell. Prot. 9, 1437-48 (2010).

8. Williamson, D. S., et al. Appl. Microbiol. Biotechnol. 88, 143-153 (2010).

9. Johnson M., et al. NAR 37(14), e98 (2009).

10. Pacheco, A., et al. Microbiol. 155, 2021-2028 (2009).

11. Wilson, A.C., et al. J. Bacteriol. 190(15), 5522-5525 (2008).

 

Notes

ACCUZYME is a trademark of Bioline.

Wielkość250 U
Aktywność 5’-3’ egzonukleazyTAK
Aktywność 3’-5’ egzonukleazyNIE
Stężenie2.5u/µl

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ACCUZYME DNA Polymerase (BIO-21051), 250 U

ACCUZYME DNA Polymerase (BIO-21051), 250 U

Dostawca: BLIRT S.A.

Cena netto, nie zawiera podatku VAT.

Użyty przelicznik: 1 euro = 4,25 zł

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